Human Biglycan Recombinant Protein
Recombinant Human BGN Protein for Signal Transduction Research
Product Information
CAT#: 0519-PROTP21810-20ug
Size: 20 µg
Expression System: Baculovirus
Tag: His-Tag
Dry Ice: No
Hazardous: No
Storage: 2°C to 8°C short-term; -20°C to -80°C long-term
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product Description
Human Biglycan Recombinant Protein is a recombinant form of human Biglycan (BGN), a small leucine-rich proteoglycan involved in extracellular matrix organization and cellular signaling. The protein is expressed using a baculovirus expression system and contains a His-tag for purification and detection.
This recombinant protein corresponds to amino acid residues 38–368 of the human Biglycan sequence. It is supplied in liquid form and characterized by SDS-PAGE purity analysis and a cell-based bioactivity assay.
Key Features
- Recombinant human Biglycan protein.
- Produced using a baculovirus expression system.
- His-tag for purification and detection.
- Protein sequence domain: amino acids 38–368.
- Purity greater than 90% by SDS-PAGE.
- Endotoxin level below 1 EU per 1 µg of protein.
- Characterized for SDS-PAGE and bioactivity applications.
Biological Background
Biglycan is an extracellular matrix proteoglycan encoded by the BGN gene. It belongs to the small leucine-rich proteoglycan family and contributes to extracellular matrix organization and interactions between cells and their surrounding environment.
Biglycan is also associated with cellular signaling processes and has been investigated in studies of tissue biology, inflammation, development, and cell-matrix interactions.
Applications
- SDS-PAGE analysis.
- Cell-based bioactivity studies.
- Signal transduction research.
- Extracellular matrix and proteoglycan research.
- Studies of cell-matrix interactions.
Technical Specifications
| Product Name | Human Biglycan Recombinant Protein |
| Catalog Number | 0519-PROTP21810-20ug |
| Product Size | 20 µg |
| Gene Name | BGN |
| UniProt Accession | P21810 |
| Synonyms | Biglycan preproprotein, BGN, DSPG1, MRLS, PG-S1, PGI, SEMDX, SLRR1A |
| Expression System | Baculovirus |
| Sequence Domain | 38–368 amino acids |
| Tag | His-Tag |
| Purity | > 90% by SDS-PAGE |
| Concentration | 0.5 mg/mL (determined by absorbance at 280 nm) |
| Observed Molecular Weight | 38.3 kDa (340 amino acids) |
| Endotoxin | < 1 EU per 1 µg of protein (LAL method) |
| Bioactivity | ED50 ≤ 20 µg/mL |
| Product Form | Liquid |
| Field of Research | Signal Transduction |
| Applications | SDS-PAGE, Bioactivity |
| Dry Ice | No |
| Hazardous | No |
Formulation
Supplied in phosphate-buffered saline (PBS), pH 7.4, containing 10% glycerol.
Storage and Handling
- Short-term storage: 2°C to 8°C for up to 1 week.
- Long-term storage: Aliquot and store at -20°C to -80°C.
- Avoid repeated freeze-thaw cycles.
- Shipping condition: Gel ice.